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Catalog Number | orb19279 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to HSP90B1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat, Zebrafish |
Dilution range | ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5 μg/ml |
Conjugation | Unconjugated |
MW | 92.5 |
Target | GPR94 / TRA1 |
Entrez | 7184 |
Protein Sequence | KEGVKFDESEKTKE |
RRID | AB_10753325 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti TRA1 antibody, anti GRP94 antibody, anti tumo Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Liver shows staining in some hepatocyte nuclei and weak cytoplasm staining of hepatocytes. Recommended concentration, 3-5ug/ml.WB: Approx 100kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 92.5kDa according to NP_003290.1). Recommended concentration: 0.01-0.03 μg/ml. |
Expiration Date | 12 months from date of receipt. |
0.5 μg/ml staining of HeLa (A) and U2OS (B), and (0.3 μg/ml) on HepG2 (C), cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminecence.
6 μg/ml staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.