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Goat anti-GPR94 / TRA1 Antibody

Catalog Number: orb19279

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19279
CategoryAntibodies
DescriptionGoat polyclonal antibody to HSP90B1
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, FC, IF, IHC, WB
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat, Zebrafish
Dilution rangeELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5 μg/ml
ConjugationUnconjugated
MW92.5
TargetGPR94 / TRA1
Entrez7184
Protein SequenceKEGVKFDESEKTKE
RRIDAB_10753325
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti TRA1 antibody, anti GRP94 antibody, anti tumo
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Liver shows staining in some hepatocyte nuclei and weak cytoplasm staining of hepatocytes. Recommended concentration, 3-5ug/ml.WB: Approx 100kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 92.5kDa according to NP_003290.1). Recommended concentration: 0.01-0.03 μg/ml.
Expiration Date12 months from date of receipt.
Goat anti-GPR94 / TRA1 Antibody

0.5 μg/ml staining of HeLa (A) and U2OS (B), and (0.3 μg/ml) on HepG2 (C), cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminecence.

Goat anti-GPR94 / TRA1 Antibody

6 μg/ml staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Goat anti-GPR94 / TRA1 Antibody

Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.

Goat anti-GPR94 / TRA1 Antibody

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.