GPR94/TRA1 Antibody

• Catalog Number: orb19279
• Category: Antibodies
• Description: Goat polyclonal antibody to HSP90B1
• Target: GPR94 / TRA1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat, Zebrafish
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KEGVKFDESEKTKE
• RRID: AB_10753325
• MW: 92.5
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Liver shows staining in some hepatocyte nuclei and weak cytoplasm staining of hepatocytes. Recommended concentration, 3-5ug/ml.WB: Approx 100kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 92.5kDa according to NP_003290.1). Recommended concentration: 0.01-0.03 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti TRA1 antibody, anti GRP94 antibody, anti tumo
• Note: For research use only
• Entrez: 7184

0.5 μg/ml staining of HeLa (A) and U2OS (B), and (0.3 μg/ml) on HepG2 (C), cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminecence.

6 μg/ml staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.