GPR81/FKSG80 Antibody

SKU: orb20248

Description

Goat polyclonal antibody to GPR81

Research Area

Epigenetics, GPCR, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 40kDa band observed in Human Liver lysates (calculated MW of 39.3kDa according to NP_115943.1). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 55kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GPR81 / FKSG80
• Protein Sequence: QQLARQARMKKATR
• Molecular Weight: 39.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GPR81 antibody, anti G protein-coupled receptor 81 antibody, anti FKSG80 antibody, anti GPR104 antibody, anti TA-GPCR antibody, anti G protein-coupled receptor 104 antibody, anti T-cell activation G protein-coupled receptor antibody, anti putative chemokine receptor antibody

orb20248 (0.5g/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

GPR81 / FKSG80

NCBI Gene ID

27198