Goat anti-GPR81 / FKSG80 Antibody

• Catalog Number: orb20248
• Category: Antibodies
• Description: Goat polyclonal antibody to GPR81
• Target: GPR81 / FKSG80
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Canine, Human, Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QQLARQARMKKATR
• RRID: AB_10749161
• MW: 39.3
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 0.3-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 40kDa band observed in Human Liver lysates (calculated MW of 39.3kDa according to NP_115943.1). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 55kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GPR81 antibody, anti G protein-coupled recept
• Note: For research use only
• Entrez: 27198

orb20248 (0.5g/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.