Goat anti-GOLGA3 Antibody

• Catalog Number: orb19091
• Category: Antibodies
• Description: Goat polyclonal antibody to GOLGA3
• Target: GOLGA3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HSQSRASKEGPGE
• RRID: AB_10749762
• MW: 167
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 170kDa band observed in human HeLa lysates (calculated MW of 167kDa according to NP_005886.2). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at appox 25kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti GOLGA3 antibody, anti HGNC:4426 antibody, ant
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 2802

orb19091 (0.1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.