Goat anti-GOLGA3 Antibody

SKU: orb19091

Description

Goat polyclonal antibody to GOLGA3

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 170kDa band observed in human HeLa lysates (calculated MW of 167kDa according to NP_005886.2). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at appox 25kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GOLGA3
• Protein Sequence: HSQSRASKEGPGE
• Molecular Weight: 167
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GOLGA3 antibody, anti HGNC:4426 antibody, anti GCP170 antibody, anti MEA-2 antibody, anti Golgi autoantigen, golgin subfamily a, 3 antibody, anti Golgi complex-associated protein of 170 kD antibody, anti Golgi membrane associated protein antibody, anti Golgi peripheral membrane protein antibody, anti SY2/SY10 protein antibody, anti golgin-160 antibody, anti golgin-165 antibody, anti male enhanced antigen-2 antibody

orb19091 (0.1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

GOLGA3

NCBI Gene ID

2802