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Catalog Number | orb195355 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to GNL3 |
Target | GNL3 (aa115-126) |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | TENKAKSGKQNS |
MW | 62.0; 60.5 |
Tested applications | ELISA, IHC, WB |
Dilution range | ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml |
Application notes | This antibody is expected to recognize both reported isoforms (NP_055181.3; NP_996561.1). Reported variants represent identical protein: NP_996561.1, NP_996562.1.WB: Approx 65kDa band observed in nuclear lysates of cell line K562 (calculated MW of 62.0kDa according to NP_055181.3). Recommended concentration: 0.03-0.1μg/ml. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | anti GNL3 antibody, anti guanine nucleotide bindin Read more... |
Note | For research use only |
Entrez | 26354 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of K562 nuclear lysate using GNL3 antibody
Immunohistochemical staining of Human Small Intestine using GNL3 antibody.