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Catalog Number | orb125075 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to GNB3 |
Target | GNB3 & GNB4 |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | SGHDNRVSCLGVT |
MW | 37.2 |
Tested applications | ELISA, IHC, WB |
Dilution range | ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5µg/ml |
Application notes | WB: Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 37.2kDa according to NP_002066.1. Recommended concentration: 0.1-0.3µg/ml.. IHC: In paraffin embedded Human Cerebellum shows strong axonal staining in the Molecular layer and less so in the Granular layer. Recommended concentration, 3-5µg/ml. |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti G protein, beta-3 subunit antibody, anti GTP- Read more... |
Note | For research use only |
Entrez | 2784, 59345 |
Western blot analysis of HepG2 lysate using GNB3 antibody (35ug)
Immunohistochemical analysis of formalin-fixed and paraffin embedded human Cerebellum tissue using GNB3 antibody