Goat anti-GNB3 & GNB4 Antibody

SKU: orb125075

Description

Goat polyclonal antibody to GNB3

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Application Notes:
  WB: Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 37.2kDa according to NP_002066.1. Recommended concentration: 0.1-0.3µg/ml.. IHC: In paraffin embedded Human Cerebellum shows strong axonal staining in the Molecular layer and less so in the Granular layer. Recommended concentration, 3-5µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GNB3 & GNB4
• Molecular Weight: 37.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti G protein, beta-3 subunit antibody, anti GTP-binding regulatory protein beta-3 chain antibody, anti guanine nucleotide binding protein (G protein), beta polypeptide 3 antibody, anti guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3 antibody, anti guanine nucleotide-binding protein, beta-3 antibody, anti GNB3 antibody

Western blot analysis of HepG2 lysate using GNB3 antibody (35ug)

Immunohistochemical analysis of formalin-fixed and paraffin embedded human Cerebellum tissue using GNB3 antibody

Quick Database Links

Gene Symbol

GNB3 & GNB4

NCBI Gene ID

59345, 2784