Goat anti-Glycophorin A Antibody

SKU: orb19105

Description

Goat polyclonal antibody to GYPA

Research Area

Cardiovascular Disease

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2000, WB: 1-3 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Human Kidney shows bright staining of erythrocytes. Recommended concentration, 2-4ug/ml.WB: Approx 18kDa band observed in human liver lysates (calculated MW of 16.4kDa according to NP_002090.2). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Glycophorin A
• Protein Sequence: SVEIENPETSDQ
• Molecular Weight: 16.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti glycophorin A (includes MN blood group) antibody, anti HGNC:4702 antibody, anti GYPA antibody, anti GPA antibody, anti GPErik antibody, anti GPSAT antibody, anti HGpMiV antibody, anti HGpMiX antibody, anti HGpMiXI antibody, anti HGpSta(C) antibody, anti MN antibody, anti MNS antibody, anti Mi.V glycoprotein (24 AA) antibody, anti erythroid-lineage-specific membrane sialoglycoprotein antibody, anti glycophorin A antibody, anti glycophorin A, GPA antibody, anti glycophorin Erik antibody, anti glycophorin MiI antibody, anti glycophorin MiV antibody, anti glycophorin MiX antibody, anti glycophorin SAT antibody, anti glycophorin Sta type C antibody, anti recombinant MiDR antibody, anti recombinant glycophorin A-B Miltenberger-DR antibody, anti CD235a antibody, anti GpMiIII antibody, anti HGpMiIII antibody, anti glycophorin A (MN blood group) antibody, anti glycophorin MiIII antibody

orb19105 (1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19105 (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

Glycophorin A

NCBI Gene ID

2993