Goat anti-Glycophorin A Antibody

• Catalog Number: orb19105
• Category: Antibodies
• Description: Goat polyclonal antibody to GYPA
• Target: Glycophorin A
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SVEIENPETSDQ
• RRID: AB_10748614
• MW: 16.4
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:2000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Human Kidney shows bright staining of erythrocytes. Recommended concentration, 2-4ug/ml.WB: Approx 18kDa band observed in human liver lysates (calculated MW of 16.4kDa according to NP_002090.2). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti glycophorin A (includes MN blood group) antib
• Research Area: Cardiovascular Disease
• Note: For research use only
• Entrez: 2993

orb19105 (1ug/ml) staining of human liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19105 (2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.