GLUT8/SLC2A8 Antibody

SKU: orb19309

Description

Goat polyclonal antibody to SLC2A8

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:32000, WB: 0.5 μg/ml
• Predicted Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 80kDa and 35kDa in Human Peripheral Blood Mononucleocyte lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 50.8kDa according to NP_055395.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Added note: The observed 35kDa band corresponds to the calculated size of 34.4kDa of EAW87663.1 (314 aa long).

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Key Properties

• Clonality: Polyclonal
• Target: GLUT8 / SLC2A8
• Protein Sequence: KGKTLEQITAH
• Molecular Weight: 50.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SLC2A8 antibody, anti solute carrier family 2, (facilitated glucose transporter) member 8 antibody, anti HGNC:13812 antibody, anti GLUT8 antibody, anti GLUTX1 antibody, anti glucose transporter X1 antibody

Quick Database Links

Gene Symbol

GLUT8 / SLC2A8

NCBI Gene ID

29988