Goat anti-GLuR5 / GRIK1 Antibody

• Catalog Number: orb19566
• Category: Antibodies
• Description: Goat polyclonal antibody to GRIK1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 104; 103
• Target: GLuR5 / GRIK1
• Entrez: 2897
• Protein Sequence: QCKQTHPTNSTS
• RRID: AB_10749923
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GLUR5 antibody, anti GRIK1 antibody, anti glu
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 150kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 104kDa according to NP_000821.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Christensen et al , J Neurosci. 2004 Oct 13;24(41):8986-93.; PMID: 15483117). Recommended concentration: 1-3 μg/ml. An additional very strong band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19566 (1ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Cortex using GRIK1 antibody