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GJC3/connexin 29 Antibody

SKU: orb20365

Description

Goat polyclonal antibody to GJC3

Images & Validation

Tested ApplicationsELISA
Dilution RangeELISA: 1:32000, WB: 0.5 μg/ml
Predicted ReactivityCanine, Human
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 60kDa band in Human Brain (Cerebellum) and in Human Skeletal Muscle lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.3kDa according to NP_853516.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetGJC3 / connexin 29
Protein SequenceLTSESTRRHKKATDS
Molecular Weight31.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti GJC3 antibody, anti gap junction protein, gamma 3, 30.2kDa antibody, anti CX30.2 antibody, anti CX31.3 antibody, anti Cx29 antibody, anti GJE1 antibody, anti connexin 29 antibody, anti connexin 30.2 antibody, anti connexin 31.3 antibody, anti gap junction protein, epsilon 1, 29kDa antibody
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Quality Guarantee

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

GJC3/connexin 29 Antibody (orb20365)

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100 μg
$ 330.00
DispatchUsually dispatched within 5-10 working days
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