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Catalog Number | orb19424 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to GJB2 |
Target | GJB2 / Connexin 26 |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Canine, Human, Mouse, Rat |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | YLLIRYCSGKSKKP |
RRID | AB_10752013 |
MW | 26.2 |
Tested applications | ELISA, FC, WB |
Dilution range | ELISA: 1:128000, WB: 0.01-1 μg/ml, IHC-P: 10ug/ml |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 26kDa band observed in Mouse Brain lysates (calculated MW of 26.4kDa according to Mouse NP_032151.1 and 26.2kDa according to Human NP_003995.2). Recommended concentration: 0.01-0.03 μg/ml. |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti GJB2 antibody, anti Connexin 26 antibody, ant Read more... |
Note | For research use only |
Entrez | 2706 |
Western blot analysis of Human Colon (Lane 1), Heart (Lane 2), Prostate (Lane 3), Skin (ane 4) and negative control Adipose (Lane 5) lysate using GJB2 antibody
Western blot analysis of Mouse Brain (Lane 1), (0.5ug/ml) Mouse Heart (Lane 2) and Rat Heart (Lane 3) lysate using GJB2 antibody
Flow cytometric analysis of HepG2 cells using GJB2 antibody