GJB2/Connexin 26 Antibody

SKU: orb19424

Description

Goat polyclonal antibody to GJB2

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.01-1 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 26kDa band observed in Mouse Brain lysates (calculated MW of 26.4kDa according to Mouse NP_032151.1 and 26.2kDa according to Human NP_003995.2). Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GJB2 / Connexin 26
• Protein Sequence: YLLIRYCSGKSKKP
• Molecular Weight: 26.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GJB2 antibody, anti Connexin 26 antibody, anti gap junction protein, beta 2, 26kDa (connexin 26) antibody, anti CX26 antibody, anti DFNA3 antibody, anti DFNB1 antibody, anti HID antibody, anti KID antibody, anti NSRD1 antibody, anti PPK antibody, anti gap junction protein beta 2 antibody, anti gap junction protein, beta 2, 26kD (connexin 26) antibody

Western blot analysis of Human Colon (Lane 1), Heart (Lane 2), Prostate (Lane 3), Skin (ane 4) and negative control Adipose (Lane 5) lysate using GJB2 antibody

Western blot analysis of Mouse Brain (Lane 1), (0.5ug/ml) Mouse Heart (Lane 2) and Rat Heart (Lane 3) lysate using GJB2 antibody

Flow cytometric analysis of HepG2 cells using GJB2 antibody

Quick Database Links

Gene Symbol

GJB2 / Connexin 26

NCBI Gene ID

2706