GIRK2/KCNJ6 Antibody

• Catalog Number: orb19650
• Category: Antibodies
• Description: Goat polyclonal antibody to KCNJ6
• Target: GIRK2 / KCNJ6
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Concentration: 2-6 μg/ml
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SSKLNQHAELET
• RRID: AB_10751569
• MW: 48.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 2-6 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 48kDa band observed in Human Brain (Hippocampus and Substantia Nigra) lysates (calculated MW of 48.5kDa according to NP_002231.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti KCNJ6 antibody, anti GIRK2 antibody, anti pot
• Research Area: Epigenetics, Neuroscience
• Note: For research use only
• Entrez: 3763

orb19650 (1ug/ml) staining of Human Brain (Substantia Nigra) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.