Goat anti-GEF5 Antibody

SKU: orb19152

Description

Goat polyclonal antibody to ARHGEF5

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 170kDa band observed in Human HeLa lysates (calculated MW of 177kDa according to NP_005426.2). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GEF5
• Molecular Weight: 177
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti GEF5 antibody, anti DKFZp686N1969 antibody, anti ARHGEF5 antibody, anti Rho guanine nucleotide exchange factor (GEF) 5 antibody, anti P60 antibody, anti TIM antibody, anti TIM1 antibody, anti guanine nucleotide regulatory protein TIM antibody, anti oncogene TIM rho guanine nucleotide exchange factor 5 antibody, anti transforming immortalized mammary oncogene antibody

orb19152 (0.1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Placenta using ARHGEF5 antibody

Quick Database Links

Gene Symbol

GEF5

NCBI Gene ID

7984