Goat anti-GEF5 Antibody

• Catalog Number: orb19152
• Category: Antibodies
• Description: Goat polyclonal antibody to ARHGEF5
• Target: GEF5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DKEIDQNSQQEE
• RRID: AB_10755900
• MW: 177
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 170kDa band observed in Human HeLa lysates (calculated MW of 177kDa according to NP_005426.2). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti GEF5 antibody, anti DKFZp686N1969 antibody, a
• Research Area: Cancer, Epigenetics
• Note: For research use only
• Entrez: 7984

orb19152 (0.1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Placenta using ARHGEF5 antibody