Goat anti-GCDFP15 / PIP Antibody

• Catalog Number: orb19224
• Category: Antibodies
• Description: Goat polyclonal antibody to PIP
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 2-3 μg/ml
• Conjugation: Unconjugated
• MW: 16.6
• Target: GCDFP15 / PIP
• Entrez: 5304
• Protein Sequence: GANKAQDNTRK
• RRID: AB_10753322
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PIP, GCDFP15, prolactin-induced protein , HGNC:899
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Breast shows staining of luminal epithelial cells of the lactiferous duct. Recommended concentration, 2-3ug/ml.WB: Approx 14kDa band observed in lysates of cell line A431 (calculated MW of 16.6kDa according to NP_002643.1). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19224 (0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19224 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.