Goat anti-GCDFP15 / PIP Antibody

SKU: orb19224

Description

Goat polyclonal antibody to PIP

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 2-3 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Breast shows staining of luminal epithelial cells of the lactiferous duct. Recommended concentration, 2-3ug/ml.WB: Approx 14kDa band observed in lysates of cell line A431 (calculated MW of 16.6kDa according to NP_002643.1). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GCDFP15 / PIP
• Molecular Weight: 16.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

PIP, GCDFP15, prolactin-induced protein , HGNC:8993, GCDFP-15, GP17, GPIP4, prolactin-inducible protein, gross cystic disease fluid protein 15

orb19224 (0.3ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19224 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

GCDFP15 / PIP

NCBI Gene ID

5304