GAPDH (Internal) Antibody

SKU: orb19168

Description

Goat polyclonal antibody to GAPDH

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.03-0.1 μg/ml, IHC-P: 2 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 36kDa band observed in Human Liver, Testes and Tonsil lysates and in Mouse Spleen lysates (calculated MW of 36.1kDa according to NP_002037.2). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GAPDH (Internal)
• Molecular Weight: 36.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti GAPDH antibody, anti glyceraldehyde-3-phosphate dehydrogenase antibody, anti HGNC:4141 antibody, anti G3PD antibody, anti GAPD antibody, anti MGC88685 antibody, anti OTTHUMP00000174431 antibody, anti OTTHUMP00000174432 antibody, anti aging-associated gene 9 protein antibody, anti glyceraldehyde 3-phosphate dehydrogenase antibody

Immunohistochemical staining of Human Liver using GAPDH antibody

Immunofluorescence analysis of HeLa cells using GAPDH antibody

WB analysis of Human Liver (A), Testes (B) and Tonsil (C) lysate protein using GAPDH antibody

WB analysis of Mouse Liver (A) and Rat Heart (B) lysate protein using GAPDH antibody

Western blot analysis of HeLa (Lane 1) and NIH3T3 (Lane 2) cell lysate using GAPDH antibody

Immunofluorescence analysis of U251 cells using GAPDH antibody

Quick Database Links

Gene Symbol

GAPDH (Internal)

NCBI Gene ID

2597