GAPDH (Internal) Antibody

• Catalog Number: orb19168
• Category: Antibodies
• Description: Goat polyclonal antibody to GAPDH
• Target: GAPDH (Internal)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: GVNHEKYDNSLK
• RRID: AB_10748645
• MW: 36.1
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.03-0.1 μg/ml, IHC-P: 2 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 36kDa band observed in Human Liver, Testes and Tonsil lysates and in Mouse Spleen lysates (calculated MW of 36.1kDa according to NP_002037.2). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti GAPDH antibody, anti glyceraldehyde-3-phospha
• Research Area: Apoptotic, Cancer, Epigenetics, Neuroscience, Sign
• Note: For research use only
• Entrez: 2597
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Liver using GAPDH antibody

Immunofluorescence analysis of HeLa cells using GAPDH antibody

WB analysis of Human Liver (A), Testes (B) and Tonsil (C) lysate protein using GAPDH antibody

WB analysis of Mouse Liver (A) and Rat Heart (B) lysate protein using GAPDH antibody

Western blot analysis of HeLa (Lane 1) and NIH3T3 (Lane 2) cell lysate using GAPDH antibody

Immunofluorescence analysis of U251 cells using GAPDH antibody