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GAPDH (C Terminus) Loading Control, biotinylated Antibody

Catalog Number: orb334083

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SizePriceQuantity
100 μg$ 500.00
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DispatchUsually dispatched within 5-10 working days
Catalog Numberorb334083
CategoryAntibodies
DescriptionGoat polyclonal antibody against GAPDH conjugated to Biotin.
TargetGAPDH (C Terminus) Loading Control, biotinylated
ClonalityPolyclonal
Species/HostGoat
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Predicted ReactivityCanine, Porcine
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Protein SequenceHQVVSSDFNSDT.
MW36.1; 31.5
Tested applicationsELISA, WB
Dilution rangeELISA: 1:64000, WB: 0.3-1 μg/ml
Application notesWB: Approx 35kDa band observed in lysates of cel line HEK293 (calculated MW of 36.1kDa according to NP_002037.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1µg/ml.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-GAPDH antibody, Anti- glyceraldehyde-3-phosph
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NoteFor research use only
Entrez2597
Expiration Date12 months from date of receipt.
GAPDH (C Terminus) Loading Control, biotinylated Antibody

Western blot analysis of HEK293 lysate using GAPDH antibody (Biotin)