GAL Antibody

SKU: orb20373

Description

Goat polyclonal antibody to Galanin

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 0.2-0.5 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  IHC: Frozen section of Human Hypothalamus shows staining of galaninergic axons in the human periventricular nucleus. Recommended concentration, 0.2-0.5μg/ml.WB: Approx 17kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 13.3kDa according to NP_057057.2). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GAL
• Molecular Weight: 13.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti-GAL 1 antibody, anti- GAL antibody, anti- GAL GMAP antibody, anti- GAL1 antibody, anti- GALA_HUMAN antibody, anti- Galanin message associated peptide antibody, anti- Galanin message-associated peptide antibody, anti- Galanin precursor antibody, anti- Galanin prepropeptide antibody, anti- Galanin related peptide antibody, anti- Galanin/GMAP prepropeptide antibody, anti- GALN antibody, anti- GLNN antibody, anti- GMAP antibody, anti- MGC40167 antibody

orb20373 Negative Control showing staining of paraffin embedded Human Small Intestine, with no primary antibody.

orb20373 (8µg/ml) staining of paraffin embedded Human Small Intestine. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb20373 (1µg/ml) staining of Mouse Brain (A) Small Intestine (B) and Stomach (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Western blot analysis of human cerebellum lysate (35µg protein in RIPA buffer) using GAL antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human hypothalamus tissue using GAL antibody

Western blot analysis of Mouse and Rat Brain lysate using Galanin antibody

Quick Database Links

Gene Symbol

GAL

NCBI Gene ID

51083