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GABRB2 Antibody

SKU: orb20138

Description

Goat polyclonal antibody to GABRB2

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA
Dilution RangeELISA: 1:4000, WB: 0.2 μg/ml
Predicted ReactivityBovine, Canine, Human, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 80kDa band in Mouse Brain lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.2kDa according to Human NP_068711.1 and 54.6kDa according to Mouse NP_032096.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetGABRB2
Protein SequenceHSFGRNALERHVAQ
Molecular Weight59.2; 54.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti GABRB2 antibody, anti gamma-aminobutyric acid (GABA) A receptor, beta 2 antibody, anti MGC119386 antibody, anti MGC119388 antibody, anti MGC119389 antibody, anti OTTHUMP00000160872 antibody

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NCBI Gene Details

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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GABRB2 Antibody (orb20138)

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Available Sizes

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100 μg
$ 330.00
DispatchUsually dispatched within 5-10 working days
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