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GABRA6 (aa15-26) Antibody

SKU: orb131691

Description

Goat polyclonal to GABRA6

Images & Validation

Tested ApplicationsELISA, IHC
Dilution RangeELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Porcine
Application Notes
Preliminary experiments gave bands at approx 75kDa and 37kDa in Human Cerebellum lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 51.0kDa according to NP_000802.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reportedWB: Preliminary experiments gave bands at approx 75kDa and 37kDa in Human Cerebellum lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 51.0kDa according to NP_000802.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetGABRA6 (aa15-26)
Protein SequenceENALGKLEVEGN
Molecular Weight51
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

GABRA6; gamma-aminobutyric acid (GABA) A receptor, alpha 6; MGC116903; MGC116904; GABA subunit A receptor alpha 6; GABA(A) receptor subunit alpha-6; gamma-aminobutyric acid receptor subunit alpha-6
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Quality Guarantee

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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GABRA6 (aa15-26) Antibody (orb131691)

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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