Fubp1 (mouse, aa160-174) Antibody

• Catalog Number: orb12321
• Category: Antibodies
• Description: Goat polyclonal to Fubp1.
• Target: Fubp1 (mouse, aa160-174)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DQIVEKGRPAPGFHH
• MW: 67.4
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 4-8 μg/ml
• Application notes: WB: Approx 75kDa band observed in lysates of cell line Jurkat (calculated MW of 67.4kDa according to NP_476513.2). Recommended concentration: 0.03-0.1µg/ml.. IHC: In paraffin embedded Human Tonsil shows staining in a selection of cells mostly outside the germinal centre. Recommended concentration: 4-8µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Fubp1 antibody
• Note: For research use only
• Entrez: 8880
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Jurkat lysate using Fubp1 antibody

Immunohistochemical staining of Human Tonsil using Fubp1 antibody