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Catalog Number | orb19747 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to FZD7 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Mouse |
Dilution range | ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 63.6 |
Target | Frizzled 7 |
Entrez | 8324 |
Protein Sequence | RFYHRLSHSSKGET |
RRID | AB_10750518 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti rizzled 7 antibody, anti frizzled homolog 7 ( Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 60kDa band observed in lysates of cell lines HEK293 and HepG2 (calculated MW of 63.6kDa according to NP_003498.1). Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19747 (0.3ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb19747 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.