Goat anti-FOXP3 / SCURFIN Antibody

SKU: orb18370

Description

Goat polyclonal antibody to FOXP3

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Tonsil, stains nuclei of some cells in the interfollicular area. Recommended concentration, 0.3-1 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FOXP3 / SCURFIN
• Molecular Weight: 47.2; 43.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti FOXP3 antibody, anti SCURFIN antibody, anti forkhead box P3 antibody, anti JM2 antibody, anti AIID antibody, anti IPEX antibody, anti PIDX antibody, anti XPID antibody, anti DIETER antibody, anti JM2 protein antibody, anti immunodeficiency, polyendocrinopathy, enteropathy, X-linked antibody, anti immune dysregulation, polyendocrinopathy, enteropathy, X-linked antibody, anti MGC141961 antibody, anti MGC141963 antibody, anti FOXP3delta7 antibody, anti scurfin antibody

orb18370 (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells. This data was

orb18370 staining of CD25-sorted (Treg) human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).

Western blot analysis of Human Muscle(lane 1), MOLT4(lane 2) and Pancreas(lane 3) lysate using FOXP3 antibody

Flow cytometric analysis of Jurkat cells using FOXP3 antibody

Immunofluorescence analysis of Human blood cells using FOXP3 antibody

Quick Database Links

Gene Symbol

FOXP3 / SCURFIN

NCBI Gene ID

50943