Goat anti-FOXP3 / SCURFIN Antibody

• Catalog Number: orb18370
• Category: Antibodies
• Description: Goat polyclonal antibody to FOXP3
• Target: FOXP3 / SCURFIN
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SQRPSRCSNPTPGP
• RRID: AB_10749065
• MW: 47.2; 43.4
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Tonsil, stains nuclei of some cells in the interfollicular area. Recommended concentration, 0.3-1 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti FOXP3 antibody, anti SCURFIN antibody, anti f
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 50943

orb18370 (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells. This data was

orb18370 staining of CD25-sorted (Treg) human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).

Western blot analysis of Human Muscle(lane 1), MOLT4(lane 2) and Pancreas(lane 3) lysate using FOXP3 antibody

Flow cytometric analysis of Jurkat cells using FOXP3 antibody

Immunofluorescence analysis of Human blood cells using FOXP3 antibody