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FOXO3A/FOXO3/FKHRL1 Antibody

SKU: orb18806

Description

Goat polyclonal antibody to FOXO3

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:8000, WB: 0.1-1 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 90kDa band observed in human Brain and Heart lysates (calculated MW of 71.3kDa according to NP_001446 and NP_963853). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetFOXO3A / FOXO3 / FKHRL1
Protein SequenceGAKQASSQSWVPG
Molecular Weight71.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration0.1-1 μg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti FOXO3A antibody, anti FKHRL1 antibody, anti AF6q21 antibody, anti FKHRL1P2 antibody, anti MGC12739 antibody, anti MGC31925 antibody, anti DKFZp781A0677 antibody, anti forkhead box O3A antibody, anti forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody, anti forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 antibody, anti FOXO3 antibody, anti forkhead box O3 antibody, anti FOXO2 antibody, anti forkhead homolog (rhabdomyosarcoma) like 1 antibody, anti forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 antibody
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FOXO3A/FOXO3/FKHRL1 Antibody

orb18806 staining (0.1ug/ml) of Human Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

FOXO3A/FOXO3/FKHRL1 Antibody

orb18806 staining (0.3µg/ml) of Human Heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

FOXO3A/FOXO3/FKHRL1 Antibody (orb18806)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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