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Catalog Number | orb18806 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to FOXO3 |
Target | FOXO3A / FOXO3 / FKHRL1 |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Concentration | 0.1-1 μg/ml |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | GAKQASSQSWVPG |
RRID | AB_10767136 |
MW | 71.3 |
Tested applications | ELISA, IHC, WB |
Dilution range | ELISA: 1:8000, WB: 0.1-1 μg/ml |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 90kDa band observed in human Brain and Heart lysates (calculated MW of 71.3kDa according to NP_001446 and NP_963853). Recommended concentration: 0.1-0.3 μg/ml. |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti FOXO3A antibody, anti FKHRL1 antibody, anti A Read more... |
Note | For research use only |
Entrez | 2309 |
orb18806 staining (0.1ug/ml) of Human Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
orb18806 staining (0.3µg/ml) of Human Heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.