Goat anti-FOXI1 / FKHL10 Antibody

• Catalog Number: orb18857
• Category: Antibodies
• Description: Goat polyclonal antibody to FOXI1
• Target: FOXI1 / FKHL10
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TSGVLYPREGTEV
• RRID: AB_10751671
• MW: 41.0; 30.8
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:2000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: This product has been successfully used in Mouse Epididymus (Blomqvist et al, EMBO J. 2006 Sep 6;25(17):4131-41. PMID 16932748).WB: Preliminary experiments gave bands at approx 50 kDa and 25 kDa in Human Kidney lysate after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted sizes of 41.0kDa according to NP_036320 (isoform a) and 30.8kDa according to NP_65892 (isofrom b). The bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti FOXI1 antibody, anti forkhead box I1 antibody
• Note: For research use only
• Entrez: 2299

orb18857 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

orb18857 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb18857 (1µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemical staining of Human Testis using FOXI1 antibody

WB analysis of Human Testes lysate using FOXI1 antibody