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Item 1 of 5
Item 1 of 5
Goat anti-FOXI1 / FKHL10 Antibody
Catalog Number: orb18857
| Catalog Number | orb18857 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to FOXI1 |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Human |
| Dilution range | ELISA: 1:2000, WB: 1-3 μg/ml |
| Conjugation | Unconjugated |
| MW | 41.0; 30.8 |
| Target | FOXI1 / FKHL10 |
| Entrez | 2299 |
| Protein Sequence | TSGVLYPREGTEV |
| RRID | AB_10751671 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti FOXI1 antibody, anti forkhead box I1 antibody Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: This product has been successfully used in Mouse Epididymus (Blomqvist et al, EMBO J. 2006 Sep 6;25(17):4131-41. PMID 16932748).WB: Preliminary experiments gave bands at approx 50 kDa and 25 kDa in Human Kidney lysate after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted sizes of 41.0kDa according to NP_036320 (isoform a) and 30.8kDa according to NP_65892 (isofrom b). The bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
| Expiration Date | 12 months from date of receipt. |
orb18857 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
orb18857 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
orb18857 (1µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemical staining of Human Testis using FOXI1 antibody
WB analysis of Human Testes lysate using FOXI1 antibody
