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Goat anti-FOXG1 / BF2 Antibody

Catalog Number: orb18407

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb18407
CategoryAntibodies
DescriptionGoat polyclonal antibody to FOXG1
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, FC, WB
ReactivityBovine, Canine, Human, Mouse, Rat
Dilution rangeELISA: 1:64000, WB: 0.3-1.0 μg/ml
ConjugationUnconjugated
MW52.4
TargetFOXG1 / BF2
Entrez2290
Protein SequenceHQNQGSSSNPLIH
RRIDAB_10750485
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti forkhead box G1 antibody, anti BF1 antibody,
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 48 kDa band observed in Human Brain lysates (calculated MW of 52.4kDa according to NP_005240.3 (FOXG1B) and 50.5kDa according to CAA55038.1 (FOXG1A). Recommended concentration: 0.3-1.0 μg/ml. An additional band of unknown identity was also consistently observed at 70kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Expiration Date12 months from date of receipt.
Goat anti-FOXG1 / BF2 Antibody

orb18407 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.