FOXG1/BF2 Antibody

• Catalog Number: orb18407
• Category: Antibodies
• Description: Goat polyclonal antibody to FOXG1
• Target: FOXG1 / BF2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HQNQGSSSNPLIH
• RRID: AB_10750485
• MW: 52.4
• Tested applications: ELISA, FC, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1.0 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 48 kDa band observed in Human Brain lysates (calculated MW of 52.4kDa according to NP_005240.3 (FOXG1B) and 50.5kDa according to CAA55038.1 (FOXG1A). Recommended concentration: 0.3-1.0 μg/ml. An additional band of unknown identity was also consistently observed at 70kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti forkhead box G1 antibody, anti BF1 antibody,
• Research Area: Developmental Biology
• Note: For research use only
• Entrez: 2290
• Expiration Date: 12 months from date of receipt.

orb18407 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.