FOXG1/BF2 Antibody

SKU: orb18407

Description

Goat polyclonal antibody to FOXG1

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1.0 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 48 kDa band observed in Human Brain lysates (calculated MW of 52.4kDa according to NP_005240.3 (FOXG1B) and 50.5kDa according to CAA55038.1 (FOXG1A). Recommended concentration: 0.3-1.0 μg/ml. An additional band of unknown identity was also consistently observed at 70kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FOXG1 / BF2
• Molecular Weight: 52.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti forkhead box G1 antibody, anti BF1 antibody, anti BF2 antibody, anti FHKL3 antibody, anti FKH2 antibody, anti FKHL1 antibody, anti FKHL2 antibody, anti FKHL3 antibody, anti FKHL4 antibody, anti FOXG1A antibody, anti FOXG1B antibody, anti FOXG1C antibody, anti HBF-1 antibody, anti HBF-2 antibody, anti HBF-3 antibody, anti HBF-G2 antibody, anti HBF2 antibody, anti HFK1 antibody, anti HFK2 antibody, anti HFK3 antibody, anti KHL2 antibody, anti QIN antibody, anti OTTHUMP00000178764 antibody, anti brain factor 2 antibody, anti forkhead box G1A antibody, anti forkhead box G1B antibody, anti forkhead box G1C antibody, anti forkhead, drosophila, homolog-like 2 antibody, anti forkhead-like 1 antibody, anti forkhead-like 2 antibody, anti forkhead-like 3 antibody, anti forkhead-like 4 antibody, anti oncogene QIN antibody

orb18407 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

FOXG1 / BF2

NCBI Gene ID

2290