Goat anti-FOXE1 / TTF2 Antibody

• Catalog Number: orb18539
• Category: Antibodies
• Description: Goat polyclonal antibody to FOXE1
• Target: FOXE1 / TTF2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: AYPGGIDRFVSAM
• RRID: AB_10746526
• MW: 38.1
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:8000, WB: 0.3 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows strong nuclear staining in developing sperm cells. Recommended concentration, 2-3ug/ml.This product has also been successfully used in Human Thyroid Gland: Zhang et al, Pathol Int. 2006 May;56(5):240-5.PMID: 166WB: Preliminary experiments gave an approx 70kDa band in Human Thymus and Thyroid Gland lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.1kDa according to NP_004464.2. The 65kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti FOXE1 antibody, anti TTF2 antibody, anti fork
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 2304

Immunohistochemical staining of Human Thyroid Gland using FOXE1 antibody

Immunohistochemical staining of Human Testis using FOXE1 antibody