Goat anti-FOXE1 / TTF2 Antibody

SKU: orb18539

Description

Goat polyclonal antibody to FOXE1

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:8000, WB: 0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows strong nuclear staining in developing sperm cells. Recommended concentration, 2-3ug/ml.This product has also been successfully used in Human Thyroid Gland: Zhang et al, Pathol Int. 2006 May;56(5):240-5.PMID: 166WB: Preliminary experiments gave an approx 70kDa band in Human Thymus and Thyroid Gland lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.1kDa according to NP_004464.2. The 65kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FOXE1 / TTF2
• Molecular Weight: 38.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti FOXE1 antibody, anti TTF2 antibody, anti forkhead box E1 (thyroid transcription factor 2) antibody, anti FOXE2 antibody, anti HFKH4 antibody, anti HFKL5 antibody, anti TITF2 antibody, anti TTF-2 antibody, anti FKHL15 antibody, anti forkhead (Drosophila)-like 15 antibody, anti forkhead box E1 antibody, anti forkhead box E2 antibody, anti thyroid transcription factor-2 antibody, anti Forkhead, drosophila, homolog-like 15 antibody

Immunohistochemical staining of Human Thyroid Gland using FOXE1 antibody

Immunohistochemical staining of Human Testis using FOXE1 antibody

Quick Database Links

Gene Symbol

FOXE1 / TTF2

NCBI Gene ID

2304