Goat anti-FLVCR Antibody

SKU: orb18933

Description

Goat polyclonal antibody to FLVCR1

Research Area

Epigenetics, Neuroscience, Stem Cells

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 59.9kDa according to NP_054772.1). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Successfully used in WB application in the publicatioon: Rey M.A. et al. Enhanced alternative splicing of the FLVCR1 gene in Diamond Blackfan anemia disrupts FLVCR1 expression and function that are critical for erythropoiesis. Haematologica 2008 Nov;93(11):1617-26. Epub 2008 Sep 24. PMID:18815190.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FLVCR
• Protein Sequence: KTVMLSKQSESAI
• Molecular Weight: 59.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FLVCR antibody, anti feline leukemia virus subgroup C cellular receptor antibody, anti FLVCR protein antibody

orb18933 (0.1ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

FLVCR

NCBI Gene ID

28982