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Catalog Number | orb18696 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to Flotillin 1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Porcine |
Dilution range | ELISA: 1:32000, WB: 0.1-0.3 μg/ml |
Conjugation | Unconjugated |
MW | 47.4; 42.1 |
Target | Flotillin 1 |
Entrez | 10211 |
Protein Sequence | SISQVNHKPLRTA |
RRID | AB_10748819 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti FLOT1 antibody, anti flotillin 1 antibody, an Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 48kDa band observed in lysates of cell line H460 (calculated MW of 47.4kDa according to NP_005794.1). In transfected HEK293 transiently expressing FLOT1 a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of H460 lysate using Flotillin 1 antibody
Western blot analysis of HEK293 lysate using Flotillin 1 antibody
Immunohistochemical staining of Human Kidney using Flotillin 1 antibody
ELISA, IHC, WB | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Bovine, Canine, Human, Porcine | |
Goat | |
Polyclonal | |
Unconjugated |