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| Catalog Number | orb18892 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to FHL1 |
| Target | Fhl1/ Slim1 (mouse) |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Mouse, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Immunogen | Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001070830.1; NP_001274729.1. |
| Protein Sequence | CNKRFVFHNEQVY |
| RRID | AB_10755541 |
| MW | 31.9; 33.6 |
| Tested applications | ELISA, WB |
| Dilution range | ELISA: 1:32000, WB: 0.05-0.3 μg/ml, IHC-P: 2 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows fibrous staining in the cytoplasm of developing sperm cells.. Recommended concentration, 2-2ug/ml.WB: Approx 30kDa band observed in Human Muscle lysates (calculated MW of 31.9kDa according to NP_001440). Recommended concentration: 0.05-0.3 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti FHL1 antibody, anti SLIM1 antibody, anti KYO- Read more... |
| Research Area | Cardiovascular Disease, Developmental Biology, Epi Read more... |
| Note | For research use only |
| Entrez | 2273 |

Western blot analysis of Human Muscle lysate using FHL1 antibody

Immunohistochemical staining of Human Testis using FHL1 antibody
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