Goat anti-FCER1A (aa177-189) Antibody

SKU: orb195356

Description

Goat polyclonal antibody to FCER1A

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Application Notes:
  WB: Approx 50kDa band observed in Human Pripheral Blood Mononucleocytes lysates (calculated MW of 29.6kDa according to NP_001992.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ganguly et al, J Biol Chem. 2007 Nov 9;282(45):32758-64.PMID: 17728245). Recommended concentration: 0.3-1μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FCER1A (aa177-189)
• Molecular Weight: 29.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti FCER1A antibody, anti Fc fragment of IgE, high affinity I, receptor for antibody, anti alpha polypeptide antibody, anti FCE1A antibody, anti FcERI antibody, anti Fc IgE receptor, alpha polypeptide antibody, anti Fc epsilon RI alpha-chain antibody, anti Fc-epsilon RI-alpha antibody, anti high affinity immunoglobulin epsilon receptor alpha-subunit antibody, anti high affinity immunoglobulin epsilon receptor subunit alpha antibody, anti igE Fc receptor subunit alpha antibody, anti immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide antibody

6 µg/ml staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Quick Database Links

Gene Symbol

FCER1A (aa177-189)

NCBI Gene ID

2205