Fascin 2 Antibody

SKU: orb19567

Description

Goat polyclonal antibody to FSCN2

Images & Validation

• Tested Applications: ELISA, ICC, IHC
• Dilution Range: ELISA: 1:64000, IHC-P: 4 μg/ml
• Reactivity: Mouse
• Predicted Reactivity: Canine, Human, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx 75kDa and 22kDa in Mouse Eye lysates after 0.05 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 57.4kDa according to NP_001070650.1 and 55.1kDa according to NP_036550.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Fascin 2
• Protein Sequence: CHHRGSNQLDTNR
• Molecular Weight: 57.4; 55.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus) antibody, anti RFSN antibody, anti RP30 antibody, anti fascin 2 antibody, anti retinal fascin antibody

4 µg/ml staining of paraffin embedded Mouse Brain. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Quick Database Links

Gene Symbol

Fascin 2

NCBI Gene ID

25794