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ETEA/UBXD8 Antibody

SKU: orb18739

Description

Goat polyclonal antibody to FAF2

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:16000, WB: 0.3-1 μg/ml
ReactivityHuman, Mouse
Predicted ReactivityBovine, Canine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 55kDa band observed in lysates of Human and Mouse Thymus and of cell line Jurkat (calculated MW of 52.6kDa according to NP_055428.1). Recommended concentration: 0.3-1µg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetETEA / UBXD8
Protein SequenceHTEVLFVQDLTDE
Molecular Weight52.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

FAF2; ETEA; UBXD8; KIAA0887; expressed in T-cells and eosinophils in atopic dermatitis; UBX domain containing 8; Fas associated factor family member 2; UBXN3B; UBX domain protein 3B

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ETEA/UBXD8 Antibody

orb18739 staining (0.3µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ETEA/UBXD8 Antibody

orb18739 (1µg/ml) staining of Human (A) and Mouse (B) Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ETEA/UBXD8 Antibody (orb18739)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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