EMI2 Antibody

SKU: orb19137

Description

Goat polyclonal antibody to FBXO43

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Human, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: This product has been successfully used on Rat Pachytene Spermatocytes showing a 71kDa band (Perrard et al, PLoS One. 2009 Oct 5;4(10):e7237. PMID: 19802389). Recommended concentration: 1-3 μg/ml.Experiment Notes: This product has been successfully used in Immunofluorescence and Flow Cytometry on Rat meiotic Testis cells: (Perrard et al, PLoS One. 2009 Oct 5;4(10):e7237. PMID: 19802389). Recommended concentration: 5-10ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: EMI2
• Molecular Weight: 78.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti FBXO43 antibody, anti EMI2 antibody, anti F-box protein 43 antibody, anti Fbx43 antibody, anti early mitotic inhibitor 2 antibody, anti ERP1 antibody

Western blot analysis of Mouse Ovary lysate using FBXO43 antibody

Quick Database Links

Gene Symbol

EMI2

NCBI Gene ID

286151