ELMO1 Antibody

SKU: orb18348

Description

Goat polyclonal antibody to ELMO1

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Zebrafish
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 1-2 μg/ml. Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa). Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ELMO1
• Protein Sequence: PKEPSNYDFVYDCN
• Molecular Weight: 83.8; 28.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ELMO1 antibody, anti engulfment and cell motility 1 (ced-12 homolog, C. elegans) antibody, anti CED12 antibody, anti KIAA0281 antibody, anti ced-12 homolog 1 antibody, anti engulfment and cell motility 1 antibody, anti CED-12 antibody, anti ELMO-1 antibody, anti MGC126406 antibody, anti OTTHUMP00000207056 antibody, anti OTTHUMP00000207060 antibody

Western blot analysis of Jurkat lysate using ELMO1 antibody.

Quick Database Links

Gene Symbol

ELMO1

NCBI Gene ID

9844