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| Catalog Number | orb20300 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to EIF5A |
| Target | eIF5A |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | RPPHRASFLKRLESK |
| RRID | AB_10753749 |
| MW | 20.2 |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 20kDa band observed in MOLT4 lysates (calculated MW of 20.2kDa according to NP_001137232.1). Recommended concentration: 1-3 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti EIF5A antibody, anti eukaryotic translation i Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 1984 |

Western blot analysis of MOLT4 lysate using EIF5A antibody

Immunohistochemical staining of Human Spleen of EIF5A antibody

Immunohistochemical staining of Human Tonsil of EIF5A antibody

Immunohistochemical staining of Human Vessel of EIF5A antibody
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