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EGLN3 Antibody

Catalog Number: orb19921

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SizePriceQuantity
100 μg$ 500.00
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DispatchUsually dispatched within 5-10 working days
Catalog Numberorb19921
CategoryAntibodies
DescriptionGoat polyclonal to EGLN3
TargetEGLN3
ClonalityPolyclonal
Species/HostGoat
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Protein SequenceRLGKYYVKERSK
RRIDAB_10753591
MW27.7
Tested applicationsELISA, WB
Dilution rangeELISA: 1:8000, WB: 0.1-0.3 μg/ml
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 30kDa band observed in Human Skeletal Muscle lysates (calculated MW of 27.7kDa according to NP_071356.1). Recommended concentration: 0.1-0.3 μg/ml.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesanti EGLN3 antibody, anti egl nine homolog 3 (C. e
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Research AreaApoptotic, Cancer, Cardiovascular Disease, DNA Dam
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NoteFor research use only
Entrez112399
Expiration Date12 months from date of receipt.
EGLN3 Antibody

orb19921 optimised QC. Primary incubation 1 hour at room temperature. Images A, B, C: Human Heart, Skin, Duodenum lysate at primary Ab concentration 0.5 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

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