EGLN3 Antibody

SKU: orb19921

Description

Goat polyclonal to EGLN3

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 30kDa band observed in Human Skeletal Muscle lysates (calculated MW of 27.7kDa according to NP_071356.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: EGLN3
• Protein Sequence: RLGKYYVKERSK
• Molecular Weight: 27.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti EGLN3 antibody, anti egl nine homolog 3 (C. elegans) antibody, anti FLJ21620 antibody, anti HIFPH3 antibody, anti MGC125998 antibody, anti MGC125999 antibody, anti PHD3 antibody, anti EGL nine (C.elegans) homolog 3 antibody, anti egl nine homolog 3 antibody, anti egl nine-like protein 3 isoform antibody

orb19921 optimised QC. Primary incubation 1 hour at room temperature. Images A, B, C: Human Heart, Skin, Duodenum lysate at primary Ab concentration 0.5 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

EGLN3

NCBI Gene ID

112399