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Catalog Number | orb19921 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to EGLN3 |
Target | EGLN3 |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Bovine, Canine, Mouse, Rat |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | RLGKYYVKERSK |
RRID | AB_10753591 |
MW | 27.7 |
Tested applications | ELISA, WB |
Dilution range | ELISA: 1:8000, WB: 0.1-0.3 μg/ml |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 30kDa band observed in Human Skeletal Muscle lysates (calculated MW of 27.7kDa according to NP_071356.1). Recommended concentration: 0.1-0.3 μg/ml. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | anti EGLN3 antibody, anti egl nine homolog 3 (C. e Read more... |
Research Area | Apoptotic, Cancer, Cardiovascular Disease, DNA Dam Read more... |
Note | For research use only |
Entrez | 112399 |
Expiration Date | 12 months from date of receipt. |
orb19921 optimised QC. Primary incubation 1 hour at room temperature. Images A, B, C: Human Heart, Skin, Duodenum lysate at primary Ab concentration 0.5 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.