Goat anti-EGFR Antibody

SKU: orb318750

Description

Goat polyclonal antibody to EGFR

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Application Notes:
  WB: Approx 170kDa band observed in lysates of cell line HeLa (calculated MW of 134kDa according to NP_005219.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: EGFR
• Protein Sequence: QKGSHQISLDNPD
• Molecular Weight: 134
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti EGFR antibody, anti epidermal growth factor receptor antibody, anti erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian antibody, anti HGNC:3236 antibody, anti ERBB antibody, anti ERBB1 antibody, anti mENA antibody, anti avian erythroblastic leukemia viral (v-erb-b) oncogene homolog antibody, anti epidermal growth factor receptor antibody, anti epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog) antibody, anti truncated epidermal growth factor receptor antibody

Western blot analysis of HeLa lysate using EGFR antibody

Quick Database Links

Gene Symbol

EGFR

NCBI Gene ID

1956