Goat anti-EGFR Antibody

• Catalog Number: orb318750
• Category: Antibodies
• Description: Goat polyclonal antibody to EGFR
• Target: EGFR
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QKGSHQISLDNPD
• MW: 134
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
• Application notes: WB: Approx 170kDa band observed in lysates of cell line HeLa (calculated MW of 134kDa according to NP_005219.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti EGFR antibody, anti epidermal growth factor r
• Note: For research use only
• Entrez: 1956
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa lysate using EGFR antibody