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Goat anti-Dysadherin Antibody
Catalog Number: orb18356
| Catalog Number | orb18356 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to FXYD5 |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Human |
| Dilution range | ELISA: 1:8000 |
| Conjugation | Unconjugated |
| MW | 19.5 |
| Target | Dysadherin |
| Entrez | 53827 |
| Protein Sequence | GKCRQLSRLCRNHCR |
| RRID | AB_10747689 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti FXYD5 antibody, anti dysadherin antibody, ant Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 38 kDa band in Human Spleen lysate and an approx 26 kDa in Human Lung lysate after 1 μg/ml antibody staining. The bands were successfully blocked by incubation with the immunizing peptide. Please note that according to the literature this protein is O-glycosylated (PMID: 12672699), which may explain the different sizes of bands we observe given the calculated size of 19.5 kDa according to NP_054883 and NP_659003. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates |
| Expiration Date | 12 months from date of receipt. |
orb18356 staining (0.5ug/ml) of Human Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
