Goat anti-DUOX1 (aa972-985) Antibody

• Catalog Number: orb137100
• Category: Antibodies
• Description: Goat polyclonal antibody to DUOX1
• Target: DUOX1 (aa972-985)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 0.1-0.3 μg/ml
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CSRSDIETELTPQR
• MW: 177
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml
• Application notes: Approx 170kDa band observed in Human Testis lysates (calculated MW of 177kDa according to NP_059130.2). Recommended concentration: 0.1-0.3 μg/ml. An additional band of unknown identity was also consistently observed at 140kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any such results been reported with other antibodies/lysates. Have any further splice variants/modified forms been reported.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti dual oxidase 1 antibody, anti flavoprotein NA
• Note: For research use only
• Entrez: 53905

Western blot analysis of Human Testis lysate (35ug protein in RIPA buffer) using DUOX1 antibody