Goat anti-DTX3 Antibody

• Catalog Number: orb20428
• Category: Antibodies
• Description: Goat polyclonal antibody to DTX3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Dilution range: ELISA: 1:8000, WB: 0.2-0.5 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 38
• Target: DTX3
• Entrez: 196403
• Protein Sequence: KNKVTVSKPVWD
• RRID: AB_10750601
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DTX3 antibody, anti deltex 3 homolog (Drosoph
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 90kDa band observed in Mouse Fetal Brain lysates and in lysates of cell line NIH3T3 (calculated MW of 38.0kDa according to Human NP_848597.1 and 37.98kDa to Mouse NP_109639.1). The observed much higher molecular weight corresponds to earlier findings in literature with different antibodies (Takeyama et al, J Biol Chem. 2003 Jun13;278(24):21930-7.; PMID: 12670957). Recommended concentration: 0.2-0.5 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20428 (0.3µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb20428 (10µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.