DTX3 Antibody

SKU: orb20428

Description

Goat polyclonal antibody to DTX3

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.2-0.5 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 90kDa band observed in Mouse Fetal Brain lysates and in lysates of cell line NIH3T3 (calculated MW of 38.0kDa according to Human NP_848597.1 and 37.98kDa to Mouse NP_109639.1). The observed much higher molecular weight corresponds to earlier findings in literature with different antibodies (Takeyama et al, J Biol Chem. 2003 Jun13;278(24):21930-7.; PMID: 12670957). Recommended concentration: 0.2-0.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DTX3
• Molecular Weight: 38
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti DTX3 antibody, anti deltex 3 homolog (Drosophila) antibody, anti FLJ34766 antibody, anti MGC138863 antibody, anti MGC138864 antibody, anti RNF154 antibody, anti deltex 3 homolog antibody

orb20428 (0.3µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb20428 (10µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

DTX3

NCBI Gene ID

196403