Goat anti-DRD5 Antibody

SKU: orb19368

Description

Goat polyclonal antibody to DRD5

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution range: ELISA: 1:128000, WB: 1-2 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 75-80kDa band in lysates of Human Brain (hippocampus), Kidney, Spleen and Thymus after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.0kDa according to NP_000789.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DRD5
• Molecular Weight: 53
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti DRD5 antibody, anti dopamine receptor D5 antibody, anti DBDR antibody, anti DRD1B antibody, anti DRD1L2 antibody, anti MGC10601 antibody, anti D1beta dopamine receptor antibody, anti dopamine receptor D1B antibody

Immunofluorescence analysis of paraformaldehyde fixed U251 cells using DRD5 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using DRD5 antibody.

Flow cytometric analysis of paraformaldehyde fixed U251 cells using DRD5 antibody.

Quick Database Links

Gene Symbol

DRD5

NCBI Gene ID

1816