Goat anti-DRD5 Antibody

• Catalog Number: orb19368
• Category: Antibodies
• Description: Goat polyclonal antibody to DRD5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 1-2 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Conjugation: Unconjugated
• MW: 53
• Target: DRD5
• Entrez: 1816
• Protein Sequence: NREVDNDEEEGPFD
• RRID: AB_10746891
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DRD5 antibody, anti dopamine receptor D5 anti
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 75-80kDa band in lysates of Human Brain (hippocampus), Kidney, Spleen and Thymus after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.0kDa according to NP_000789.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed U251 cells using DRD5 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using DRD5 antibody.

Flow cytometric analysis of paraformaldehyde fixed U251 cells using DRD5 antibody.