Goat anti-DRD5 Antibody

• Catalog Number: orb19368
• Category: Antibodies
• Description: Goat polyclonal antibody to DRD5
• Target: DRD5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NREVDNDEEEGPFD
• RRID: AB_10746891
• MW: 53
• Tested applications: ELISA, FC, IF
• Dilution range: ELISA: 1:128000, WB: 1-2 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 75-80kDa band in lysates of Human Brain (hippocampus), Kidney, Spleen and Thymus after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.0kDa according to NP_000789.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DRD5 antibody, anti dopamine receptor D5 anti
• Research Area: Neuroscience
• Note: For research use only
• Entrez: 1816

Immunofluorescence analysis of paraformaldehyde fixed U251 cells using DRD5 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using DRD5 antibody.

Flow cytometric analysis of paraformaldehyde fixed U251 cells using DRD5 antibody.