Doublecortex/DCX Antibody

SKU: orb422484

Description

Goat polyclonal antibody to DCX

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.003-0.01 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Feline, Rat
• Application Notes:
  WB: Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6kDa according to Mouse NP_001103692.1). Recommended concentration: 0.003-0.01µg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 7µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5µg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: Doublecortex / DCX
• Protein Sequence: KASPTPQKTSAKSPG
• Molecular Weight: 49.3; 40.5; 40.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DCX ; doublecortex; lissencephaly; X-linked (doublecortin) ; RP5-914P14.1; DBCN; DC; LISX; SCLH; XLIS ; OTTHUMP00000062892; doublecortin; doublin; lissencephalin-X

Quick Database Links

Gene Symbol

Doublecortex / DCX

NCBI Gene ID

1641