Dnajc5 (aa177-191) Antibody

• Catalog Number: orb12269
• Category: Antibodies
• Description: Goat polyclonal antibody to Dnajc5
• Target: Dnajc5 (aa177-191)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SATETTQLTADSHPS
• MW: 22.1
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.5-1.5 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 20kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 22.1kDa according to NP_058055.1). Recommended concentration: 0.5-1.5µg/ml. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.. IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti 2610314I24Rik antibody, anti AU018536 antibod
• Note: For research use only
• Entrez: 80331

orb12269 (0.5µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Skeletal Muscle using Dnajc5 antibody