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Catalog Number | orb12269 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to Dnajc5 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:64000, WB: 0.5-1.5 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 22.1 |
Target | Dnajc5 (aa177-191) |
Entrez | 80331 |
Protein Sequence | SATETTQLTADSHPS |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti 2610314I24Rik antibody, anti AU018536 antibod Read more... |
Note | For research use only |
Application notes | WB: Approx 20kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 22.1kDa according to NP_058055.1). Recommended concentration: 0.5-1.5µg/ml. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.. IHC: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml. |
Expiration Date | 12 months from date of receipt. |
orb12269 (0.5µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of Human Skeletal Muscle using Dnajc5 antibody