DKK4 Antibody

SKU: orb18362

Description

Goat polyclonal antibody to DKK4

Research Area

Developmental Biology, Epigenetics, Neuroscience, Signaling Pathways, Stem Cells, Wnt

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 30-35kDa band seen in Jurkat lysate. Recommended for use at 1-3 μg/ml. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa [form (i)], 30-32 kDa [form (ii)] and 15-17 kDa [form (iii)]. We believe this antibody will recognise 30-32kDa species of DKK4.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DKK4
• Protein Sequence: RQHARLRVCQKIEKL
• Molecular Weight: 24.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti DKK4 antibody, anti dickkopf homolog 4 (Xenopus laevis) antibody, anti DKK-4 antibody, anti hdkk-4 antibody, anti Dickkopf gene 4 antibody, anti dickkopf (Xenopus laevis) homolog 4 antibody, anti MGC129562 antibody, anti MGC129563 antibody, anti dickkopf homolog 4 antibody

orb18362 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

DKK4

NCBI Gene ID

27121