Goat anti-DKK3 / REIC Antibody

• Catalog Number: orb18368
• Category: Antibodies
• Description: Goat polyclonal antibody to DKK3
• Target: DKK3 / REIC
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: AAAALLGGEEI
• RRID: AB_10749064
• MW: 38.4
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 70kDa band observed in lysates of cell line HEK293 (calculated MW of 38.4kDa according to NP_037385.2, NP_056965.3 and NP_001018067.1). The observed molecular weight corresponds to earlier findings in literature (Krupnik et al, Gene. 1999 Oct 1;238(2):301-13.; PMID: 10570958 ). Recommended concentration: 0.03-0.1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DKK3 antibody, anti REIC antibody, anti dickk
• Research Area: Developmental Biology, Epigenetics, Neuroscience,
• Note: For research use only
• Entrez: 27122

Western blot analysis of HEK293 lysate using DKK3 antibody

Immunohistochemical staining of Human Cortex using DKK3 antibody