Dispatched homolog 1 Antibody

• Catalog Number: orb18636
• Category: Antibodies
• Description: Goat polyclonal to Dispatched homolog 1
• Target: Dispatched homolog 1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SELSGESLLIKTL
• RRID: AB_10751655
• MW: 171; 144.7
• Tested applications: ELISA, FC, IF
• Dilution range: ELISA: 1:32000, IF/ICC: 2.5ug/ml, FACS: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave no signal but low background in Human Lung and A549 extracts at up to 1 μg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysatesExperiment Notes: Immunofluorescence: Cell membrane staining in cell line U87. These data corresponds to earlier findings in literature with different antibodies (Etheridge et al, Development. 2010 Jan;137(1):133-40; PMID: 20023168).
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DISP1 antibody, anti DISPA antibody, anti MGC
• Note: For research use only
• Entrez: 84976

immunofluoroscence analysis of U87 cells (2.5 ug/ml) using Dispatched homolog 1 antibody

Flow cytometric analysis of paraformaldehyde-fixed HEK293 cells using Dispatched homolog 1 antibody.