Goat anti-Dicarbonyl Reductase Antibody

• Catalog Number: orb18673
• Category: Antibodies
• Description: Goat polyclonal to Dicarbonyl Reductase
• Target: Dicarbonyl Reductase
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: GSTLPVEGGFWAC
• RRID: AB_10750732
• MW: 25.9
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 1-2µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded human kidney, stains membrane / cytoplasm in epithelial cells of renal glomeruli and tubules. Recommended concentration, 1-2ug/ml.WB: Approx 26kDa band observed in human kidney, liver and testes lysates (calculated MW of 25.9kDa according to NP_057370.1). Recommended for use at 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DCXR antibody, anti dicarbonyl/L-xylulose red
• Note: For research use only
• Entrez: 51181
• Expiration Date: 12 months from date of receipt.

orb18673 staining (0.1ug/ml) of human kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18673 (1ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.