Delta-Sarcoglycan (aa245-257) Antibody

• Catalog Number: orb125144
• Category: Antibodies
• Description: Goat Polyclonal to SGCD. NP_000328.2; NP_758447.1; NP_001121681.1, PMID: 17993586 and GeneID: 6444.
• Target: Delta-Sarcoglycan (aa245-257)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RLPHGSYTPTGTR
• MW: 32.2; 28.8; 32.1
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5µg/ml
• Application notes: WB: Approx 37kDa band observed in Human and Rat Heart lysates (calculated MW of 32.2kDa according to NP_000328.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3µg/ml.. IHC: In paraffin embedded Human Skeletal Muscle shows sarcolemmar staining of the fibres. Recommended concentration, 3-5µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SGCD antibody, anti sarcoglycan, delta (35kDa
• Note: For research use only
• Entrez: 6444

Western blot analysis of human heart lysate using SGCD antibody (35ug)

Immunohistochemical analysis of formalin-fixed and paraffin embedded human skeletal muscle tissue using SGCD antibody