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Catalog Number | orb19217 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to DCP1A |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse, Rat |
Dilution range | ELISA: 1:16000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
MW | 63.3 |
Target | DCP1A |
Entrez | 55802 |
Protein Sequence | QVLTKNKDNHN |
RRID | AB_10755555 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti DCP1A antibody, anti decapping enzyme antibod Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 75kDa band observed in human lysates of A431 and HeLa (calculated MW of 63.3kDa according to NP_060873.3). Recommended concentration: 1-3 μg/ml. An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot. |
Expiration Date | 12 months from date of receipt. |
orb19217 (2ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.