DBP5/DDX19 Antibody

SKU: orb19584

Description

Goat polyclonal antibody to DDX19B

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:4000, WB: 0.5-1.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 50kDa band observed in lysates of human ovary and of cell line K562 (calculated MW of 50.5kDa according to NP_001014451.1). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DBP5 / DDX19
• Protein Sequence: DLPVDKDGNPDNETY
• Molecular Weight: 53.9; 50.5; 41.8; 54.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti DDX19 antibody, anti DBP5 antibody, anti DDX19B antibody, anti DEAD (Asp-Glu-Ala-As) box polypeptide 19B antibody, anti RNAh antibody, anti ATP-dependent RNA helicase DDX19 antibody, anti DEAD (Asp-Glu-Ala-As) box polypeptide 19 antibody, anti DEAD-box RNA helicase DEAD5 antibody, anti DEAD-box protein 5 antibody, anti DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 antibody, anti DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog) antibody, anti yeast Dbp5 homolog antibody

orb19584 (0.5ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

DBP5 / DDX19

NCBI Gene ID

11269, 55308