DAZL Antibody

• Catalog Number: orb19110
• Category: Antibodies
• Description: Goat polyclonal antibody to DAZL
• Target: DAZL
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Human, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: GNGPQKKSVDR
• RRID: AB_10755758
• MW: 33.2
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Mouse Testis shows cytoplasm staining of spermatogonia. Additional staining is observed in the cytoplasm of elongatng spermatids but this additional staining is not in agreement with results obtained by another antibody (Ruggiu et alWB: Approx 35kDa band observed in human ovary and human testes lysates (calculated MW of 33.2kDa according to NP_001342.2). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DAZL antibody, anti deleted in azoospermia-li
• Research Area: Developmental Biology, Epigenetics, Stem Cells
• Note: For research use only
• Entrez: 1618

orb19110 (0.1ug/ml) staining of human ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of Rat Testes lysate using DAZL antibody

Immunohistochemical staining of Mouse Testis lysate using DAZL antibody